首页|The order of immunoglobulin light chain kappa and lambda usage in primary and secondary lymphoid tissues of germ-free and conventional piglets

The order of immunoglobulin light chain kappa and lambda usage in primary and secondary lymphoid tissues of germ-free and conventional piglets

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  • NSTL
  • Elsevier
In pigs (Sus scrofa), the initial immunoglobulin rearrangement of the kappa light chain is replaced by lambda before the heavy chains rearrange, and the light chains may rearrange even later. This study investigates whether these developmental differences are reflected in the usage of IGK and IGL genes. We found large differences between peripheral B cells and those developing in the bone marrow, and between B cells in germ-free piglets and conventional pigs. During early B cell development in the bone marrow, more 3' V and 5'J gene segments for both light chains are used. However, in the peripheral naive repertoire, more 5' IGLV and 3' IGLJ genes are used. A similar shift toward the use of more 5' IGKV and 3' IGKJ genes is observed later after antigen exposure in conventional pigs. The expression profile showed that most lambda(+) B cells are generated earlier, while kappa+ B cells develop from late precursors that already contain the lambda rearrangement. The initial lambda rearrangement is retained in both lambda(+) and kappa(+) B lymphocytes, and multiple lambda transcripts can be found in individual cells. The overall pool of the IGLV repertoire is therefore much larger and more diversified than for IGKV. The kappa repertoire is further restricted to the preferential use of only two major IGKV genes, reflecting the limitation for only two consecutive rearrangements. Tracing of silenced lambda transcripts in kappa(+) B cells further confirmed the unconventional mechanism of differential rearrangements in pigs. Our results underline the diversity of the immune system among mammals.

Porcine immune systemB cell developmentImmunoglobulin rearrangementImmunoglobulin light chainsB cell receptorsLymphocyte differentiationANTIBODY REPERTOIRE DEVELOPMENTB-CELL LYMPHOGENESISILEAL PEYERS-PATCHESDELTA T-LYMPHOCYTESGENE REARRANGEMENTSNEONATAL PIGLETSFETALEXPRESSIONORGANIZATIONDIVERSITY

Stepanova, Katerina、Sinkorova, Jana、Srutkova, Dagmar、Sinkora Jr, Marek、Sinkora, Simon、Splichal, Igor、Splichalova, Alla、Butler, John E.、Sinkora, Marek

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Czech Acad Sci

Univ Iowa

2022

10.1016/j.dci.2022.104392

Developmental and Comparative Immunology

Developmental and Comparative Immunology

SCI
ISSN：0145-305X
年,卷(期)：2022.131
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