High-performance affinity chromatography for characterization of human immunoglobulin G digestion with papain

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外文摘要：Reactive continuous rods of macroporous poly(glycidyl methacrylate-co-ethylene dimethacrylate) were prepared within the confinies of a stainless steel column. Then papain was immobilized on these monoliths either directly or linked by a spacer arm. In a further step, a protein A affinity column was used for the characterization of the digestion products of human immunoglobulin G (IgG) by papain. The results showed that papain immobilized on the monolithic rod through a spacer arm exhibits higher activity for the digestion of human IgG than that without a spacer arm. The apparent Michaelis-Menten kinetic constants of free and immobilized papain, K_m and V_(max), were determined. The digestion conditions of human IgG with free and immobilized papain were optimized. Comparison of the thermal stability of free and immobilized papain showed that the immobilized papain exhibited higher thermal stability than the free enzyme. The half-time of immobilized papain reaches about a week under optimum pH and temperature conditions.

外文关键词：

monolithic rodsimmunoglobulin Gpapain

作者：

Quanzhou Luo、Xiqin Mao、Liang Kong、Xiaodong Huang、Hanfa Zou

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作者单位：

National Chromatographic R & A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 161 Zhongshan Road, Dalian 116011, China

出版年：

2002

Journal of Chromatography, Biomedical Applications

ISSN：0378-4347

年,卷(期)：2002.776(2)