Tetrahedron letters2022，Vol.934.DOI:10.1016/j.tetlet.2022.153696

Biocatalytic assembly of chemically modified oligonucleotides

Stanger, Frederic, V Peschke, Theo Schroer, Kirsten Mann, Greg Nhat Quang Nguyen Trung Burrer, Nathalie Grassinger, Franziska Piccioni, Lorenzo Erdogan, Oemer Heimburger, Jeremy Koehl, Herve Ertle, Heike Denay, Regis Dreier, Philippe Schlama, Thierry
Tetrahedron letters2022，Vol.934.DOI:10.1016/j.tetlet.2022.153696
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  • NSTL
  • Elsevier

Biocatalytic assembly of chemically modified oligonucleotides

Stanger, Frederic, V 1Peschke, Theo 1Schroer, Kirsten 2Mann, Greg 1Nhat Quang Nguyen Trung 1Burrer, Nathalie 2Grassinger, Franziska 1Piccioni, Lorenzo 1Erdogan, Oemer 1Heimburger, Jeremy 1Koehl, Herve 1Ertle, Heike 2Denay, Regis 2Dreier, Philippe 1Schlama, Thierry1
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作者信息

  • 1. Novartis Pharma AG
  • 2. Novartis Inst BioMed Res
  • 折叠

Abstract

With renewed interest in therapeutic oligonucleotides, and at the same time the pharmaceutical industry set itself ambitious sustainability goals, there is a strong interest to investigate next-generation synthesis methods for these highly modified oligonucleotides. We herein report an RNA ligase catalyzed assembly of multiple, short oligonucleotides in aqueous solution to generate an siRNA product. A conversion of the non-native substrates to the desired product of 40 - 80 % was achieved. (c) 2022 Elsevier Ltd. All rights reserved.

Key words

Oligonucleotides/Biocatalysis/Sustainable/Ligation/RNA

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出版年

2022
Tetrahedron letters

Tetrahedron letters

CCR
ISSN：0040-4039
被引量1
参考文献量11
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