首页|Molecular cloning and functional characterization of RIP1 in large yellow croaker Larimichthys crocea
Molecular cloning and functional characterization of RIP1 in large yellow croaker Larimichthys crocea
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NSTL
Elsevier
? 2022 Elsevier LtdReceptor interacting protein 1 (RIP1) plays important roles not only in cell-death pathways but also in host innate immune responses. In the present study, a RIP1 ortholog named Lc-RIP1 was cloned and characterized in large yellow croaker (Larimichthys crocea). The open reading frame (ORF) of Lc-RIP1 is 2,046 bp, encoding a protein of 681 amino acids (aa), with an N-terminal kinase domain, an RHIM domain, and a C-terminal death domain. Subcellular localization analysis revealed that Lc-RIP1 was a cytosolic protein, which was broadly expressed in examined tissues/organs, and could be up-regulated under poly I:C, LPS, PGN, and Pseudomonas plecoglossicida stimulation in vivo based on qRT-PCR analysis. Notably, Lc-RIP1 could induce NF-κB, but not IRF3, IRF7 or type I IFN promoter activation. In addition, Lc-RIP1 overexpression could enhance Lc-MAVS, Lc-TRAF3, and Lc-TRAF6 mediated NF-κB promoter activation, and also Lc-TRIF and Lc-MAVS mediated IRF3 promoter activation, whereas suppress Lc-TRIF mediated NF-κB and type I IFN promoter activation, as well as Lc-TRAF3 and Lc-IRF3 mediated IRF3 promoter activation, Lc-IRF3 mediated type I IFN promoter activation and Lc-IRF7 mediated IRF7 promoter activation. These results collectively indicated that Lc-RIP1 function importantly in regulation of host innate immune signaling.
Large yellow croakerMAVSRIP1TRAF3TRAF6TRIF
Zou P.F.、Wang Y.L.、Li K.Q.、Shen Y.J.、Li Y.、Zhang Z.P.
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Key Laboratory of Healthy Mariculture for the East China Sea Ministry of Agriculture and Rural Affairs Fisheries College Jimei University
College of the Environment and Ecology Xiamen University
Key Laboratory of Estuarine Ecological Security and Environmental Health Tan Kah Kee College Xiamen University
College of Animal Science Fujian Agriculture and Forestry University
NSTL
Elsevier
