首页|Phytochemical and anti-oxidant evaluation of in vitro and in vivo propagated plants of Curculigo orchioides
Phytochemical and anti-oxidant evaluation of in vitro and in vivo propagated plants of Curculigo orchioides
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NSTL
Springer Nature
Curculigo orchioides (Kali Musli) is a medicinal plant commonly used in the Indian and Chinese traditional medicine system to treat jaundice and asthma. It is also used as an aphrodisiac in several countries. C. orchioides is treated as an essential resource in many pharmaceutical industries as a source of bioactive compounds. Indiscriminate use, depletion of its natural habitat, and its seasonal availability have resulted in its rarity. As per the IUCN status, Curculigo orchioides is now listed as an endangered plant. The present micropropagation study resolves its seasonal availability by ensuring a continuous supply of plants. In vitro somatic embryos were induced on the leaf explants of C. orchioides in one-fourth-strength Murashige and Skoog (MS) media. The embryos developed into whole plants in 8 wk. The in vitro plants produced were evaluated for their phytochemical evaluation and anti-oxidant activity along with their wild counterparts as controls. Both sets, wild plants termed in vivo and tissue-cultured plants called in vitro plants, were subjected to phytochemical extraction using a solvent gradient method. The extracts were analyzed qualitatively and quantitatively for phytochemical constituents. The anti-oxidant property of the extracts was screened using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging and reducing power assays. The in vivo aqueous root extract (AR) and aqueous leaf extract (AL) had similar DPPH radical scavenging activity for both in vivo and in vitro plants. In vitro methanolic root extracts showed a significantly higher DPPH radical scavenging activity than other in vitro root extracts. The study demonstrates the medicinal properties of in vitro plants conclusively. It could be effectively used as an alternative, time-saving method for sustainable, season-independent usage of endangered plants for extraction while conserving and protecting their diversity and habitat in nature.
NSTL
Springer Nature
