首页|Deuterated polyunsaturated fatty acids inhibit photoirradiation-induced lipid peroxidation in lipid bilayers

Deuterated polyunsaturated fatty acids inhibit photoirradiation-induced lipid peroxidation in lipid bilayers

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  • NSTL
  • Elsevier
Lipid peroxidation (LPO) plays a key role in many age-related neurodegenerative conditions and other disorders. Light irradiation can initiate LPO through various mechanisms and is of importance in retinal and dermatological pathologies. The introduction of deuterated polyunsaturated fatty acids (D-PUFA) into membrane lipids is a promising approach for protection against LPO. Here, we report the protective effects of D-PUFA against the photodynamically induced LPO, using illumination in the presence of the photosensitizer trisulfonated aluminum phthalocyanine (AlPcS3) in liposomes and giant unilamellar vesicles (GUV), as assessed in four experimental models: 1) sulforhodamine B leakage from liposomes, detected with fluorescence correlation spectroscopy (FCS); 2) formation of diene conjugates in liposomal membranes, measured by absorbance at 234 nm; 3) membrane leakage in GUV assessed by optical phase-contrast intensity observations; 4) UPLC-MS/MS method to detect oxidized linoleic acid (Lin)-derived metabolites. Specifically, in liposomes or GUV containing H-PUFA (dilinoleyl-sn-glycero-3-phosphatidylcholine), light irradiation led to an extensive oxidative damage to bilayers. By contrast, no damage was observed in lipid bilayers containing 20% or more D-PUFA (D2-Lin or D10-docosahexanenoic acid). Remarkably, addition of tocopherol increased the dye leakage from liposomes in HPUFA bilayers compared to photoirradiation alone, signifying tocopherol's pro-oxidant properties. However, in the presence of D-PUFA the opposite effect was observed, whereby adding tocopherol increased the resistance to LPO. These findings suggest a method to augment the protective effects of D-PUFA, which are currently undergoing clinical trials in several neurological and retinal diseases that involve LPO.

Lipid peroxidationD-PUFAPUFAMembranePhotosensitized oxidationalpha-TocopherolTocopherol-Mediated PeroxidationTroloxSynergismAge-related diseasesAMDSun protectionPhotodynamicFLUORESCENCE CORRELATION SPECTROSCOPYTOCOPHEROL-MEDIATED PEROXIDATIONPEAK INTENSITY ANALYSISALPHA-TOCOPHEROLVITAMIN-EGIANT VESICLESPHOSPHOLIPID-MEMBRANESOXIDATIVE STRESSPORE FORMATIONBINDING

Firsov, A. M.、Franco, M. S. F.、Chistyakov, D., V、Goriainov, S., V、Sergeeva, M. G.、Kotova, E. A.、Fomich, M. A.、Bekish, A., V、Sharko, O. L.、Shmanai, V. V.、Itri, R.、Baptista, M. S.、Antonenko, Y. N.、Shchepinov, M. S.

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Lomonosov Moscow State Univ

Univ Sao Paulo

SREC PFUR Peoples Friendship Univ Russia

Natl Acad Sci

Retrotope Inc

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2022

10.1016/j.jphotobiol.2022.112425

Journal of Photochemistry and Photobiology

Journal of Photochemistry and Photobiology

ISSN：1011-1344
年,卷(期)：2022.229
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