首页|In Vitro Development of Interspecific Hybrids and Isozymic Confirmation of Hybridity in Cotton
In Vitro Development of Interspecific Hybrids and Isozymic Confirmation of Hybridity in Cotton
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NSTL
In vitro development of interspecific hybrids was studied involving four Indian varieties viz; Vikas and LH 900 (Gossypium hirsutum), Lohit and BD-5 (G. arboreum) and two wild species, namely G. australe and G. bickii Cultivated varieties were used asfemale parents and their stigmas were smeared with modified Taylor's medium before pollination. Successful development of hybrids could be achieved in two interspecific crosses viz; Lohit x G. australe and BD-5 x G. australe. Effect of smearing stigmaswith Taylor's medium was found to be worth, as smearing enhanced the recovery of hybrid embryos to a greater extent. The leaves of interspecific hybrids were intermediate in shape than those of the parents and resembled more with female parents as havinglob ed structure. The length and breadth of the leaves of hybrids in both the crosses were exceeded over the parents. The length of petiole was also exceeded over the parents. Vie hybridity was confirmed through isozymic analysis. Esterase and peroxidase banding pattern and intensity in hybrids was intermediate to their parents.
NSTL
