首页|Optimized amplification of BK polyomavirus in urine

Optimized amplification of BK polyomavirus in urine

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  • NSTL
  • Elsevier
? 2021BK polyomavirus (BKPyV) is a ubiquitous pathogen that typically results in asymptomatic infection. However, in immunocompromised individuals, BKPyV viral shedding in the urine can reach 109 copies per mL. These high viral levels within urine provide ideal samples for next-generation sequencing to accurately determine BKPyV genotype and identify mutations associated with pathogenesis. Sequencing data obtained can be further analyzed to better understand and characterize the genetic diversity present in BKPyV. Here, methods are described for the successful extraction of viral DNA from urine and the subsequent amplification methods to prepare a sample for next-generation sequencing.

BK polyomavirus (BKPyV)DiversityGenotypeRolling circle amplificationVariation

Odegard E.A.、Meeds H.L.、Blackard J.T.、Kleiboeker S.B.、Ziady A.、Sabulski A.、Jodele S.、Davies S.M.、Seif A.E.、Laskin B.L.

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Division of Digestive Diseases University of Cincinnati College of Medicine

Eurofins Viracor Laboratories Lee's Summit

Divsion of Pulmonary Medicine Cincinnati Children's Hospital Medical Center

Department of Pediatrics University of Cincinnati College of Medicine

Perelman School of Medicine University of Pennsylvania

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2022

10.1016/j.jviromet.2021.114319

Journal of Virological Methods

Journal of Virological Methods

SCI
ISSN：0166-0934
年,卷(期)：2022.299
  • 16