首页|Isolation of industrially valuable alpha-Cedrol from essential oil of Platycladus orientalis (Thuja orientalis) leaves using linear gradient counter current chromatography
Isolation of industrially valuable alpha-Cedrol from essential oil of Platycladus orientalis (Thuja orientalis) leaves using linear gradient counter current chromatography
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NSTL
Elsevier
alpha-Cedrol, a monoterpene alcohol, is an industrially valuable compound used in different productions due to its diverse pharmacological and therapeutic effects. This paper reports the isolation of this compound from essential oil of Platycladus orientalis (Thuja orientalis) leaves using high performance countercurrent chromatography (HPCCC) for the first time. Two different methodologies were developed with hexane-ethyl acetate-methanol-water (HEMWat) solvent systems used in reversed phase linear gradient elution mode. In the first method, the gradient from HEMWat 17 (1/1/1/1) to 27 (19/1/19/1) solvent systems allowed isolation of 29.8 mg of high purity alpha-cedrol crystals with 86.6% recovery from 50 mg/mL sample solution as well as isolation of other components. In the 2nd method gradient from HEMWat 23 (4/1/4/1) and 27 (19/1/19/1) solvent systems of narrower polarity range resulted in doubling sample concentration to 100 mg/mL. Also the 2nd method reduced the elution time of alpha-cedrol to just 70 min with comparatively higher amount (65.72 mg) and percent recovery (94.58%) as compared to previous run.
NSTL
Elsevier
