首页|In situ decellularization of tissues to resolve the tumour-associated matrix
In situ decellularization of tissues to resolve the tumour-associated matrix
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NSTL
The extracellular matrix (ECM) is a vast, enormously complex structure made of proteins and glycans that shapes and supports all organs while enforcing cell behaviour. As cancer develops, a warped ECM, resulting from tumour-driven remodelling, promotes cancer cell invasion and metastasis. Understanding the tumour microenvironment (TME) requires a precise representation of the ECM; however, the three-dimensional arrangement of ECM components across healthy tissues and tumours is a poorly explored field. Native ECM, used as a biomaterial for cancer bioengineering, can yield invaluable information about its role during key steps of cancer progression. However, one obstacle impeding ECM mapping is its exquisite sensitivity to chemical denaturation: fixatives and clearing methods can alter ECM structure and result in single-cell resolution imaging, which is insufficient for detailed ECM exploration. I developed in situ decellularization to address both tumour deconstruction and reassembly. Tumours have an irregular vasculature, but it is possible to shunt vascular flow towards the area containing the lesion and perfuse decellularizing reagents to isolate ECM scaffolds that conserve their spatial integrity.
Alejro E. Mayorca-Guiliani
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Biotech Research and Innovation Center, University of Copenhagen, Copenhagen, Denmark.
NSTL
