首页|Magnetic immunochromatographic assay with smartphone-based readout device for the on-site detection of zearalenone in cereals

Magnetic immunochromatographic assay with smartphone-based readout device for the on-site detection of zearalenone in cereals

扫码查看
原文链接
  • NSTL
  • Elsevier
In this study, a portable and quantitative immunochromatographic assay (ICA) was developed with a smartphone-based detection device as readout. The carboxyl group coated Fe3O4 nanoparticles (MNPs) were synthesized by a one pot method and used as the labeled tracer. Initially, zearalenone monoclonal antibody (ZEN mAb) and MNPs conjugates were prepared and applied as the detection probe. MNPs probe produced brown band at the detection zone of the ICA. To increase the sensitivity, MNPs probe were used to enrich ZEN from sample solution, a portable detection device was designed and a user-friendly APP was self-written to quantify results. The cut-off value of the MNPs-ICA for ZEN in cereals was 2.5 mu g/kg. The detection limit and quantification limit were 0.06 and 0.13 mu g/kg, respectively. The recoveries ranged from 82.0 to 117.7%, with coefficient of variation from 1.3 to 10.6%. The parallel analysis results of 20 naturally contaminated cereal samples by LC-MS/MS showed that the proposed method has high accuracy. Moreover, the results of stability experiments revealed that the ICA products had a shelf life of up to 6 months. Although the feasibility is demonstrated using ZEN as a model analyte, this approach can be easily developed to be a universal analysis system and applied to detection of a wide variety of small molecule hazard factor. This study proposed a qualitative and quantitative analysis device for the food safety analysis.

ZearalenoneMagnetic nanoparticlesImmunochromatographic assaySmartphone detection deviceCerealsLATERAL FLOW IMMUNOASSAYPERFORMANCE LIQUID-CHROMATOGRAPHYLINKED-IMMUNOSORBENT-ASSAYHIGHLY SENSITIVE DETECTIONRAPID DETECTIONMAIZENANOPARTICLEDERIVATIVESAFLATOXINPRODUCTS

Li, Xiangmei、Hua, Qicheng、Liu, Zhiwei、Wang, Jin、Liang, Zaoqing、Zhou, Zexuan、Shen, Xing、Lei, Hongtao

展开 >

South China Agr Univ

2022

10.1016/j.foodcont.2021.108760

Food Control

Food Control

SCI
ISSN：0956-7135
年,卷(期)：2022.134
  • 7
  • 54