A simple MCR-Click strategy for the construction of pH-sensitive fluorescent kinase inhibitors by scaffolding a coumarin core with an indole or oxazolone moiety is described. The fluorescent probes thus obtained showed click on fluorescence useful for imaging pH-sensitive tumors, and inhibitor properties against cancer causing cyclin dependent kinase, CDK2. The optical and biological properties observed were rationalized based on DFT calculations and bioassays. The selective binding of these probes with CDK2 and their cytotoxicity towards HeLa cells without affecting normal cells demonstrates that the methodology described here is potentially useful for the design of dual functional probe cum inhibitors for imaging and therapy. (c) 2022 Elsevier Ltd. All rights reserved.
Key words
Kinase inhibitors/Click on fluorescence/Molecular docking/Hela cells/MCR-click strategy/GENERAL-METHOD/LIVE-CELL/BIOLOGICAL EVALUATION/DERIVATIVES/INDOLE/FLUOROPHORES
NSTL
Elsevier