Abstract
We developed a continuous flow system using ^-transaminase covalently immobilized on an epoxy resin modified with ethylenediamine(EDA)to synthesize(S)-l-Boc-3-aminopiperidine,which is a key intermediate for several novel inhibitors.A second functional group(-NH_3~+)was introduced to rapidly adsorb ω-transaminase,which was further rigidified by multipoint covalent attachment to the modified resin with high binding efficiency(>95%)and activity recovery(75%).The resulting immobilized ω-transaminase was stable with 90% residual activity after 15 reuse cycles.We synthesized(S)-l-Boc-3-aminopiperidine using this continuous flow system,and 95% conversion was obtained within 10 min of residence.The system was continuously operated for 24 h and achieved a space-time yield of 930.73 g·L~(-1)·day~(-1),indicating outstanding reaction efficiency.This efficient continuous bioprocess has potential for large-scale production of(S)-l-Boc-3-aminopiperidine.
NSTL