首页|Hydrophilic arginine-functionalized mesoporous polydopamine-graphene oxide composites for glycopeptides analysis

Hydrophilic arginine-functionalized mesoporous polydopamine-graphene oxide composites for glycopeptides analysis

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  • NSTL
  • Elsevier
Considering the importance of glycopeptides in the clinical diagnosis of cancer and some serious diseases, the identification of glycopeptides from complex biological samples has attracted considerable attention. Effective pre-enrichment before mass spectrometry analysis plays an important role. In this work, a kind of hydrophilic two-dimensional composites (denoted as GO@MPDA@Arg) based on mesoporous polydopamine-graphene oxide were used to selectively enrich glycopeptides in biological samples. The mesoporous polydopamine (MPDA) layer self-assembled with template Pluronic F127 provided more binding sites to load arginine, and bound arginine enhanced the hydrophilicity of the material. As a result, GO@MPDA@Arg composites exhibited excellent enrichment performance for glycopeptides, containing good selectivity (IgG digests : BSA digests = 1:50, molar ratio), low detection limit for IgG digests (10 fmol mu L-1), high loading capacity for IgG digests (200 mu g mg(-1)), and good size exclusion (IgG digests : IgG : BSA = 1:100:100, mass ratio). In addition, mouse brain tissue was selected as the actual biological sample to further study the enrichment effect of GO@MPDA@Arg composites. In three parallel experiments, a total of 401 glycopeptides belonging to 233 glycoproteins were enriched from 200 mu g digestion of mouse brain extract. The enrichment results demonstrate that GO@MPDA@Arg composites have application potential for glycopeptides enrichment in protein post-translational modification research.

Glycopeptide enrichmentElectrostatic repulsion-hydrophilic interaction & nbsp& nbspchromatography (ERLIC)ArginineMesoporous self-assemblyTissueHYDROPHILIC INTERACTION CHROMATOGRAPHYPROTEIN GLYCOSYLATIONLIQUID-CHROMATOGRAPHYEFFICIENT ENRICHMENTGLYCOPROTEINSNANOCOMPOSITESEXTRACTIONACID

Zheng, Yu、Pu, Chenlu、Zhao, Hongli、Gu, Qinying、Zhu, Tianyi、Lan, Minbo

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East China Univ Sci & Technol

2022

10.1016/j.jchromb.2021.123049

Journal of chromatography

Journal of chromatography

ISSN：1570-0232
年,卷(期)：2022.1189
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