首页|Apoptosis-induction effect of epsilon-poly-lysine against Staphylococcus aureus and its application on pasteurized milk
Apoptosis-induction effect of epsilon-poly-lysine against Staphylococcus aureus and its application on pasteurized milk
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NSTL
Elsevier
epsilon-poly-lysine (epsilon-PL) is a safe and excellent natural preservative. This study aimed to investigate the antibacterial actions of epsilon-PL against Staphylococcus aureus (S. aureus) and its anti-S. aureus activity on the pasteurized milk. Electron microscopy observation showed epsilon-PL severely disrupted the cell wall and membrane of S. aureus, resulting in the cell structure loss of the bacteria. The exposure of S. aureus to epsilon-PL obviously decreased the transmembrane potential and caused the K+ ions leakage of S. aureus. Flow cytometry analysis confirmed the bacterial membrane damage by epsilon-PL and showed the negative correlation between membrane damage and cell viability (control: 77.10% and 20.80% for viable and membrane damaged cells, 0.4 mg/mL epsilon-PL-treated group: 9.72% and 89.40% for viable and membrane damaged cells). Agarose electrophoresis and circular dichroism spectrum results demonstrated that epsilon-PL interacted with bacterial DNA via intercalative binding. And epsilon-PL also stimulated reactive oxygen species generation of S. aureus and induced the bacterial cell apoptosis. Besides, epsilon-PL could effectively inactivate or kill S. aureus in pasteurized milk during the 4 degrees C storage period (0-5 days).
NSTL
Elsevier
