首页|Smectite, sepiolite, and palygorskite for inactivation of pyocyanin, a biotoxin produced by drug-resistant Pseudomonas aeruginosa
Smectite, sepiolite, and palygorskite for inactivation of pyocyanin, a biotoxin produced by drug-resistant Pseudomonas aeruginosa
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NSTL
Elsevier
? 2021Pyocyanin is an important virulence factor in the resistance of Pseudomonas aeruginosa to antibiotics. This study evaluated the potential of three clay minerals (smectite, sepiolite, and palygorskite) in binding and degrading pyocyanin. A combination of adsorption experiments, in-situ variable-temperature x-ray diffraction (VTXRD), energy dispersive x-ray spectrometry (EDS), and UV spectroscopy were employed to investigate the (1) adsorption affinity (K) and capacity (Qmax) of clay minerals for pyocyanin, (2) binding sites for adsorption on clay minerals and (3) the degradation/transformation of pyocyanin on clay surfaces. Adsorption experiments were performed at pH3, 4.9, and 7 at which pyocyanin occurred as ～100% protonated, ～50% protonated and zwitterionic, and ～100% zwitterionic species, respectively. Overall, the estimated Qmax was 0.23-0.53 mol/kg for sepiolite, 0.21-0.43 mol/kg for palygorskite, and 0.43-0.68 mol/kg for montmorillonite. The Qmax and K were pH dependent: pH3 < pH7 for sepiolite, pH3 > pH7 for palygorskite, and pH3≈pH4.9>pH7 for montmorillonite. Pyocyanin was mainly adsorbed in the tunnels of sepiolite, external surface of palygorskite, and the interlayer of montmorillonite. The clays did not degrade pyocyanin, instead the structure was slightly transformed on the surfaces of sepiolite and montmorillonite. These results demonstrated the potentials of clay minerals in disarming P. aeruginosa through adsorbing the virulence factor pyocyanin.
NSTL
Elsevier
