首页|UPLC/ESI-Q-TOF-MS analysis of (poly)phenols, tocols and amino acids in Chaenomeles leaves versus in vitro anti-enzyme activities
UPLC/ESI-Q-TOF-MS analysis of (poly)phenols, tocols and amino acids in Chaenomeles leaves versus in vitro anti-enzyme activities
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NSTL
Elsevier
The aim of this study was to investigate the content of phenolics, free amino acids, tocopherols, tocotrienols and in vitro biological activities (alpha-amylase, alpha-glucosidase, and pancreatic lipase inhibition activity) of 12 cultivars of Chaenomeles x superba, japonica and speciosa leaves. In the present work 39 phenolic compounds were tentatively identified, including polymeric procyanidins > flavan-3-ols > phenolic acids > flavonols > flavanones > flavones > ellagitannins. Chaenomeles leaves contained on average 16.8 g of phenolics per 100 g dr y weight (dw), with C. x superba & PRIME;Nicoline & PRIME; displaying the highest (27.5 g/100 g dw), and C. x superba & PRIME;Colou r Trail & PRIME; the lowest (7.7 g/ 100 g dw) total phenolic content. Chaenomeles leaves possess a l l eight tocol analogs (fou r tocopherols (TP), and four tocotrienols (TT)), ranked in decreasing content: alpha-TP > beta-TP > gamma-T P > delta-TP > alpha-TT > beta-TT > delta-TT > gamma-TT. The most active form (alpha-tocopherol) was present in amounts from 480.6 to 7201.1 mu g/100 g dw. 23 amino acids were identified, including a l l nine essential amino acids. The highest amount of O-phospho-L-serine was found (average 249.2 mg/100 g dw). Leaves of C. japonica & PRIME;Red Joy & PRIME; displayed the highest total amino acid content (2735.2 mg/100 g dw). Chaenomeles leaves showed alpha-amylase (8.2-24.5 mg/mL), alpha-glucosidase (1.2-6.3 mg/mL), and pancreatic lipase (7.0-10.5 mg/mL) inhibition activity (expressed as ha l f maximal inhibitor y concen-tration, IC50). Chaenomeles leaves can be a good material for obtaining extracts rich in phenolics, vitamin E or amino acids, providing an alternative, natural source of these compounds to help meet the growing demand for nutraceuticals and supplements.
Japanese quinceTocopherolsTocotrienolsVitamin Ealpha-amylasealpha-glucosidasePHENOLIC-COMPOUNDSIDENTIFICATIONANTIOXIDANTNUTRACEUTICALSTOCOPHEROLSPOLYPHENOLSEXTRACTS
Wojdylo, Aneta、Tkacz, Karolina、Nowicka, Paulina、Turkiewicz, Igor Piotr
NSTL
Elsevier
