首页|3-Amino-4-hydroxybenzoic acid production from glucose and/or xylose via recombinant Streptomyces lividans

3-Amino-4-hydroxybenzoic acid production from glucose and/or xylose via recombinant Streptomyces lividans

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  • NSTL
  • Microbiol Res Foundation
The aromatic compound 3-amino-4-hydroxybenzoic acid(3,4-AHBA)can be employed as a raw material for high-performance industrial plastics.The aim of this study is to produce 3,4-AHBA via a recombinant Streptomyces lividans strain containing gril and griH genes derived from Streptomyces griseus using culture medium with glucose and/or xylose,which are the main components in lignocel-lulosic biomass.Production of 3,4-AHBA by the recombinant S.lividans strain was successful,and the productivity was aifected by the kind of sugar used as an additional carbon source.Metabolic profiles revealed that L-aspartate-4-semialdehyde(ASA),a precursor of 3,4-AHBA,and coenzyme NADPH were supplied in greater amounts in xylose medium than in glucose medium.Moreover,cultivation in TSB medium with a mixed sugar(glucose/ xylose)was found to be effective for 3,4-AHBA production,and optimal conditions for efficient production were designed by changing the ratio of glucose to xylose.The best productivity of 2.70 g/L was achieved using a sugar mixture of 25 g/L glucose and 25 g/L xylose,which was 1.5 times higher than the result using 50 g/L glucose alone.These results suggest that Streptomyces is a suitable candidate platform for 3,4-AHBA production from lignocel-lulosic biomass-derived sugars under appropriate culture conditions.

3-amino-4-hydroxybenzoic acidStreptomyces lividanssugar mixturemetabolic profile

Satoko Niimi-Nakamura、Hideo Kawaguchi、Kouji Uematsu

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Department of Chemical Science and Engineering,Graduate School of Engineering,Kobe University,1-1 Rokkodai-cho,Nada-ku,Kobe,Hyogo 657-8501,Japan

Graduate School of Science,Technology and Innovation,Kobe University,1-1 Rokkodai-cho,Nada-ku,Kobe,Hyogo 657-8501,Japan

2022

10.2323/jgam.2022.06.001

The Journal of General and Applied Microbiology

The Journal of General and Applied Microbiology

ISSN：0022-1260
年,卷(期)：2022.68(2)
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