首页|SdiA plays a crucial role in stress tolerance of C. sakazakii CICC 21544
SdiA plays a crucial role in stress tolerance of C. sakazakii CICC 21544
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NSTL
Elsevier
This study aimed to select for the quorum sensing receptor protein of C. sakazakii CICC 21544, and then evaluate the role of the receptor protein in stress tolerance. SdiA was identified as the receptor by sequence alignment. C. sakazakii CICC 21544 wild type (WT), the sdiA mutant strain (Delta sdiA), and the sdiA complemented strain (sdiA(+)) were subjected to heat treatment at 55 degrees C in tryptic soy broth (TSB) or reconstituted infant formula (RIF) and the results suggested that sdiA facilitated C. sakazakii to withstand thermal stress both in TSB and RIF. Similarly, the survival rate of Delta sdiA was significantly lower than that of WT and sdiA(+) under osmotic pressure of 12% NaCl or desiccation conditions. Acid tolerance assay showed that loss of sdiA decreased the ability of C. sakazakii to grow in simulated gastric fluid. The survivability of the three strains under combined stress conditions was also evaluated in this study. After heating, evaporation, and drying stresses, Delta sdiA was undetectable, while WT and sdiA(+) survived the treatments. This study demonstrates that sdiA contributes to the survival of C. sakazakii under different environmental stresses, and provides a basis for the sdiA-targeted inhibition of C. sakazakii in food.
Cronobacter sakazakiiQuorum sensing receptorsdiAEnvironmental stressPowdered infant formula
NSTL
Elsevier
