Free radical research2021，Vol.55Issue(1/12) ：12.DOI:10.1080/10715762.2020.1857376

Systemic RNA oxidation can be used as a biomarker of infection in challenged with Vibrio parahaemolyticus

Ya-Ya,Pian Jing-Jing,Nie Chen-Chen,Wang Qian,Liu Zhen,Liu Li-Qun,Zhang Qiu-Geng,Ou-Yang Guo-Qing,Fan Lv-Tao,Zeng Ya-Min,Dang Ya-Qing,Ma Wei,Zhang Zhen-xiang,Gao Ji-Hong,Hu Jian-Ping,Cai
Free radical research2021，Vol.55Issue(1/12) ：12.DOI:10.1080/10715762.2020.1857376
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  • Taylor & Francis

Systemic RNA oxidation can be used as a biomarker of infection in challenged with Vibrio parahaemolyticus

Ya-Ya,Pian 1Jing-Jing,Nie 1Chen-Chen,Wang 2Qian,Liu 3Zhen,Liu 2Li-Qun,Zhang 2Qiu-Geng,Ou-Yang 3Guo-Qing,Fan 2Lv-Tao,Zeng 2Ya-Min,Dang 2Ya-Qing,Ma 2Wei,Zhang 4Zhen-xiang,Gao 1Ji-Hong,Hu 1Jian-Ping,Cai2
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作者信息

  • 1. National Center for Clinical Laboratories, Beijing Hospital, National Center of Gerontology
  • 2. The Key Laboratory of Geriatrics, Beijing Institute of Geriatrics, Beijing Hospital, National
  • 3. Department of Pharmacy, Wenzhou Medical University
  • 4. Department of Pathology, National Center of Gerontology, Beijing Hospital
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Abstract

Abstract More and more evidence support the concept that RNA oxidation plays a substantial role in the progress of multiple diseases; however, only a few studies have reported RNA oxidation caused by microbial pathogens. Urinary 8-oxo-7,8-dihydroguanosine (8-oxo-Gsn) and 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxo-dGsn), which are broadly used as indicators of oxidative damage of RNA and DNA, were analyzed in this study to determine which can be used as a biomarker of infection in challenged with Vibrio parahaemolyticus (V. parahaemolyticus). In this work, 24 specific-pathogen-free (SPF) male SD rats were randomly divided into two groups: an infection group and a phosphate-buffered saline (PBS) control group. Our results proved that 8-oxo-Gsn rather than 8-oxo-dGsn was significantly increased after challenged with V. parahaemolyticus in urine and tissue samples of SD rats compared with the PBS control group. Simultaneously, white blood cells (WBCs) counts, intestinal inflammation and inflammatory factors (including CRP, IL-6, IL-1β, TNF-α, IL-10, and IL-17A) were also increased sharply. Which has more clinical value is that the trend of urinary 8-oxo-Gsn was consistent with WBCs, intestinal inflammation and all kinds of inflammatory factors. More importantly is that urinary 8-oxo-Gsn of infection group was positively correlated with WBCs and various inflammatory cytokines. In a word, our results demonstrated that as a systemic RNA oxidation biomarker, we hope 8-oxo-Gsn can be used as a biomarker of the severity of microbial pathogens infection, rather than a specific biomarker of microbial pathogens infection.

Key words

Vibrio parahaemolyticus/RNA oxidation/biomarkers/cytokines/inflammation

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出版年

2021
Free radical research

Free radical research

SCI
ISSN：1071-5762
参考文献量29
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