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Reply to: On gene silencing by the X10-23 DNAzyme
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In their recent communication, Taylor and Holliger argue that the gene silencing activity observed for the X10-23 DNAzyme is primarily the result of an antisense mechanism that is promoted by RNase H rather than the intrinsic catalytic activity of the enzyme1. The evidence they offer in support of their criticism derives from RNA cleavage assays performed under cell-free conditions that compare the 10-23 and X10-23 DNAzymes to unpublished FANAzymes (Fzl2B and Fzl66) from their laboratory. Despite several requests, the authors declined to share their unpublished work (cited as ref.4 of their comment), which was needed to evaluate the biochemical properties of their reagents.
Robert C. Spitale、John C. Chaput
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Departments of Pharmaceutical Sciences, University of California, Irvine, CA, USA
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