首页|Antigenicity and immunogenicity of HA2 and M2e influenza virus antigens conjugated to norovirus-like, VP1 capsid-based particles by the SpyTag/SpyCatcher technology
Antigenicity and immunogenicity of HA2 and M2e influenza virus antigens conjugated to norovirus-like, VP1 capsid-based particles by the SpyTag/SpyCatcher technology
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NSTL
Elsevier
Virus-like particles (VLPs) modified through different molecular technologies are employed as delivery vehicles or platforms for heterologous antigen display. We have recently created a norovirus (NoV) VLP platform, where two influenza antigens, the extracellular domain of matrix protein M2 (M2e) or the stem domain of the major envelope glycoprotein hemagglutinin (HA2) are displayed on the surface of the NoV VLPs by SpyTag/SpyCatcher conjugation. To demonstrate the feasibility of the platform to deliver foreign antigens, this study examined potential interference of the conjugation with induction of antibodies against conjugated M2e peptide, HA2, and NoV VLP carrier. High antibody response was induced by HA2 but not M2e decorated VLPs. Furthermore, HA2elicited antibodies did not neutralize the homologous influenza virus in vitro. Conjugated NoV VLPs retained intact receptor binding capacity and self-immunogenicity. The results demonstrate that NoV VLPs could be simultaneously used as a platform to deliver foreign antigens and a NoV vaccine.
NorovirusVirus-like particleVLPPlatformVaccineHA2M2eInfluenza virusSpyTagSpyCatcherBLOOD GROUP ANTIGENSESCHERICHIA-COLIVACCINEANTIBODIESPROTEINHEMAGGLUTININPROTECTIONPLATFORMBINDINGDESIGN
Heinimaki, Suvi、Lampinen, Vili、Tamminen, Kirsi、Hankaniemi, Minna M.、Malm, Maria、Hytonen, Vesa P.、Blazevic, Vesna
NSTL
Elsevier
