首页|Improvements in the extraction of milk phospholipids from beta-serum using ultrasound prior to tertiary amine extraction
Improvements in the extraction of milk phospholipids from beta-serum using ultrasound prior to tertiary amine extraction
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NSTL
Elsevier
We report improvements in the extraction of milk phospholipids (MPLs) from beta-serum, a dairy byproduct, by applying ultrasound prior to a tertiary amine extraction. Three acoustic intensities (15.53 +/- 1.20, 31.76 +/- 2.46, or 44.56 +/- 3.47 W cm(-2)) were applied for 4 min before the amine extraction (N,N-dimethylcyclohexylamine, CyNMe2). The extracted lipids were fractionated by solid phase-microextraction, and the recovered MPLs were quantified by HPLC-CAD. An acoustic intensity of 44.56 +/- 3.47 W cm(-2) followed by CyNMe2 extraction (12/1, solvent to ratio) yielded 69.67 +/- 3.45% of MPLs, while only 7.57 +/- 0.59% were recovered without ultrasound. The fraction of MPLs was made of phosphatidylinositol (32%), phosphatidylethanolamine (30%), and sphingomyelin (37%). Scanning electron images and particle size revealed significant disruption of the complex arrangement between membrane proteins and MPLs, which may help to release the MPLs into the aqueous medium.
NSTL
Elsevier
