High-performance liquid chromatographic assay validation of Manumycin A in mouse plasma

扫码查看

原文链接

NSTL

外文摘要：Manumycain A is a natural antibiotic produced by Streptomyces parvulus that has antineoplastic activity against a variety of human cancers in nude mouse models. We have developed a highly sensitive reverse phase high-performance liquid chromatography (HPLC) method based on ultraviolet (UV) detection for the determination of manumycin A in mouse plasma. Manumycin A was isolated from mouse plasma by solid-phase extraction. A gradient elution of methanol and 0.05 M H_3 PO_4 with 0.2% triethylamine mobile phase was employed and separation was achieved with a C_(18) analytical column. Manumycin A was detected by UV absorption at 345 nm. Retention time for manumycin A was 8.9 ± 0.2 min. The manumycin A peak was baseline resolved, with the nearest peak at 1.5 min distance and no interfering peaks detected. Inter- and intra-day coefficients of variance were less than 6.1 and 5.1%, respectively. Based on an extracted manumycin A standard plasma sample of 0.25 μg/ml, the assay precision was 99.8% with a mean accuracy of 95.1%. At plasma concentrations of 0.5 and 5 μg/ml, the mean recovery rates of manumycin A were 59/64 and 60./28%, respectively. The lower limit of detection (LLD) for manumycin A was 0.1 μg/ml in mouse plasma. the lower limit of quantification (LLQ) for manumycin A was 0.125 μg/ml. Results of the stability study indicated that when frozen at -80 ℃, manumycin A was stable in mouse plasma for up to 2 weeks. This method is useful in quantification of manymycin A in mouse plasma for clinical pharmacology studies in mice.

外文关键词：

manumycin A

作者：

Joanne Gonzales、Sai-Ching Jim Yeung、Judith A. Smith

展开 >

作者单位：

College of Pharmacy, University of Houston, Houston, TX, USA

Department of General Internal Medicine and Department of Endocrine Neoplasia and Hormonal Disorders, department of Medical Specialties, University of Teas M.D. Anderson Cancer Center, Houston, TX, USA

Division of Pharmacy, University of Texas M.D. Anderson Cancer Center, 1515 Holcombe Boulevard, P.O. Box 401, Houston, TX 77030-4009, USA

出版年：

2002

Journal of Chromatography, Biomedical Applications

ISSN：0378-4347

年,卷(期)：2002.776(2)