首页|Effects of new antiseizure drugs on seizure activity and anxiety-like behavior in adult zebrafish

Effects of new antiseizure drugs on seizure activity and anxiety-like behavior in adult zebrafish

扫码查看
原文链接
  • NSTL
  • Elsevier
Several studies with larvae and adult zebrafish have shown that old and new antiseizure drugs (ASDs) produce discrepant results in seizure tests, locomotor activity or anxiety models. In this study, the pentylenetetrazole seizure test (PTZ) was performed to assess the effectiveness of four new ASDs: lamotrigine (LTG), topiramate (TPM), felbamate (FBM), and levetiracetam (LEV) in the subsequent stages of seizures in adult fish. All ASDs were administered intraperitoneally (i.p.). The time of maximal anticonvulsant effect and the dose-response relationship of the drugs were assessed. The effects of studied ASDs on the locomotor activity and the anxiety-like behavior in the color preference test were also investigated. Furthermore, drug concentrations in zebrafish homogenates were determined. LTG, TPM, and LEV significantly increased the seizure latency at three subsequent stages of seizures (SI-SIII), while FBM was effective only at SI. Locomotor activity decreased after TPM treatment. TPM and FBM exhibited a strong anxiolytic-like effect in the color preference test. LEV at the highest dose tested had a weak anxiolytic-like effect. The HPLC analysis showed average concentrations of the studied ASDs in the fish body during their maximum anticonvulsant activity. The present study shows that FBM cannot inhibit all subsequent PTZ seizure stages in the adult fish. Except for LTG, the studied drugs affected the anxiety-like behavior of treated animals. Furthermore, only TPM significantly changed locomotion parameters. Our findings support the need to accurately characterize the efficacy of new ASDs at different stages of the PTZinduced seizures in adult zebrafish.

ZebrafishSeizurePentylenetetrazoleNew ASDsColor&nbsppreference

Wlaz, Piotr、Pierog, Mateusz、Socala, Katarzyna、Doboszewska, Urszula、Wyska, Elzbieta、Guz, Leszek、Szopa, Aleksandra、Serefko, Anna、Poleszak, Ewa

展开 >

Marie Curie Sklodowska Univ, Inst Biol Sci, Dept Anim Physiol & Pharmacol, Akad 19, PL-20033 Lublin

Jagiellonian Univ, Med Coll, Dept Phammcokinet & Phys Pharm, Med 9, PL-30688 Krakow, Poland

Univ Life Sci, Inst Biol Bases Anim Dis, Dept Fish Dis & Biol, Akad 12, PL-20033 Lublin, Poland

Med Univ Lublin, Chair & Dept Appl & Social Pharm, Lab Preclin Testing, Chodzki 1, PL-20093 Lublin

展开 >

2021

10.1016/j.taap.2021.115655

Toxicology and Applied Pharmacology

Toxicology and Applied Pharmacology

SCI
ISSN：0041-008X
年,卷(期)：2021.427
  • 2
  • 97