首页|Real‐time tissue perfusion assessment using fluorescence imaging topography scanning system: A preclinical investigation
Real‐time tissue perfusion assessment using fluorescence imaging topography scanning system: A preclinical investigation
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NSTL
Wiley
Abstract Background and Objectives We previously developed a real‐time fluorescence imaging topography scanning (RFITS) system for intraoperative multimodal imaging, image‐guided surgery, and dynamic surgical navigation. The RFITS can capture intraoperative fluorescence, color reflectance, and surface topography concurrently and offers accurate registration of multimodal images. The RFITS prototype is a promising system for multimodal image guidance and intuitive 3D visualization. In the current study, we investigated the capability of the RFITS system in intraoperative fluorescence vascular angiography for real‐time assessment of tissue perfusion. Study Design/Materials and Methods We conducted ex vivo imaging of fluorescence perfusion in a soft casting life‐sized human brain phantom. Indocyanine green?(ICG) solutions diluted in dimethyl sulfoxide (DMSO) and human serum were injected into the brain phantom through the vessel simulating tube (2?±?0.2?mm inner diameter) by an adjustable flow peristaltic pump. To demonstrate the translational potential of the system, an ICG/DMSO solution was perfused into blood vessels of freshly harvested porcine ears (n?=?9, inner diameter from 0.56?to 1.27?mm). We subsequently performed in vivo imaging of fluorescence‐perfused vascular structures in rodent models (n?=?10). 5?mg/ml ICG solutions prepared in sterile water were injected via the lateral tail vein. All targets were imaged by the RFITS prototype at a working distance of 350?400?mm. Results 3D visualization of 10?μg/ml ICG‐labeled continuous moving serum in the brain phantom was obtained at an average signal‐to‐background ratio (SBR) of 1.74?±?0.03. The system was able to detect intravenously diffused fluorescence in porcine tissues with an average SBR of 2.23?±?0.22. The RFITS prototype provided real‐time monitoring of tissue perfusion in rats after intravenous (IV) administration of ICG. The maximum fluorescence intensity (average SBR?=?1.94?±?0.16, p?<?0.001) was observed at Tpeak of ~30?seconds?after the ICG signal was first detected (average SBR?=?1.19?±?0.13, p?<?0.01). Conclusions We have conducted preclinical studies to demonstrate the feasibility of applying the RFITS system in real‐time fluorescence angiography and tissue perfusion assessment. Our system provides fluorescence/color composite images for intuitive visualization of tissue perfusion with 3D perception. The findings pave the way for future clinical translation.
NSTL
Wiley
