首页|Optimization of magnetic bead-based nucleic acid extraction for SARS-CoV-2 testing using readily available reagents

Optimization of magnetic bead-based nucleic acid extraction for SARS-CoV-2 testing using readily available reagents

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  • NSTL
  • Elsevier
? 2021The COVID-19 pandemic has highlighted the need for generic reagents and flexible systems in diagnostic testing. Magnetic bead-based nucleic acid extraction protocols using 96-well plates on open liquid handlers are readily amenable to meet this need. Here, one such approach is rigorously optimized to minimize cross-well contamination while maintaining sensitivity.

ContaminationCOVID-19Cross-wellHamilton NIMBUSMagnetic beadsNucleic acid extractionRNASARS-CoV-2

Haile S.、Pandoh P.K.、Smailus D.E.、Pleasance S.、Wong A.、Zhao Y.、Krzywinski M.、Nelson J.、Mungall A.J.、Jones S.J.M.、Coope R.J.、Marra M.A.、Hirst M.、Nikiforuk A.M.、Manges A.R.、Twa D.D.W.、Nguyen J.、Eisler D.、Tsang F.、Jassem A.、Prystajecky N.、Krajden M.、Moksa M.、Cao Q.、Wong M.、Su E.、Prentice L.M.

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Canada's Michael Smith Genome Sciences Centre

School of Population and Public Health University of British Columbia

BC Cancer Research Centre

British Columbia Centre for Disease Control Public Health Laboratory

Department of Microbiology and Immunology Michael Smith Laboratories University of British Columbia

Provincial Laboratory Medicine Services Provincial Health Services Authority

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2022

10.1016/j.jviromet.2021.114339

Journal of Virological Methods

Journal of Virological Methods

SCI
ISSN：0166-0934
年,卷(期)：2022.299
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