首页|Phospholipase D8 and H2S increase the production of NADPH oxidase-dependent H2O2 to respond to osmotic stress-induced stomatal closure in Arabidopsis thaliana
Phospholipase D8 and H2S increase the production of NADPH oxidase-dependent H2O2 to respond to osmotic stress-induced stomatal closure in Arabidopsis thaliana
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NSTL
Elsevier
Osmotic stress is one of the main stresses that seriously affects the survival of plants, destroying normal cell activities, and potentially leading to plant death. Phospholipase D (PLD), a major lipid hydrolase, hydrolyzes membrane phospholipids to produce phosphatidic acid (PA) and responds to many abiotic stresses. Hydrogen sulfide (H2S) emerges as the third gaseous signaling molecule involved in the complex network of signaling events. Hydrogen peroxide (H2O2) plays a crucial role as a signaling molecule in plant development and growth, and responds to various abiotic and biotic stresses. In this study, the functions and the relationship of PLD8, H2S, and H2O2 in osmotic stress-induced stomatal closure were explored. By using the seedlings of ecotype (WT), PLDo-deficient mutant (pldo), L-cysteine desulfhydrase (LCD)-deficient mutant (lcd), and pldolcd double mutant, atrbohD, and atrbohF mutant as materials, and the stomatal aperture were analyzed. The relative water loss of pldo, lcd, and pld delta lcd was higher than that of WT. Exogenous PA and NaHS could partially alleviate the leaf wilting and yellowing phenotypes of pld delta, lcd, and pld delta lcd under osmotic stress, but the mutants could not be restored to the same phenotype as WT. The fluorescence intensity of H2O2 in guard cells of pld delta, lcd, and pld delta lcd was lower than that of WT, indicating that PLD delta and LCD were involved in the production of H2O2 in guard cells. Exogenous application of H2O2 to WT, pld delta, lcd, and pld delta lcd significantly induced stomatal closure under osmotic stress. Exogenous NaHS induced stomatal closure of WT, but could not induce stomatal closure of atrbohD and atrbohF under osmotic stress. These results suggest that the accumulation of H2O2 was essential to induce stomatal closure under osmotic stress, and PLD delta and LCD acted upstream of H2O2 .
Osmotic stressPLD deltaH2SH2O2Stomatal closureArabidopis thalianaOXYGEN SPECIES ROSHYDROGEN-SULFIDENITRIC-OXIDEPLANT-RESPONSESDROUGHT-STRESSD-DELTAPEROXIDEALPHA
Liu, Qin、Liu, Ruirui、Zhou, Yaping、Wang, Wei、Wu, Guofan、Yang, Ning
NSTL
Elsevier
