首页|Investigating the role of BN-domains of FlhF involved in flagellar synthesis in Campylobacter jejuni
Investigating the role of BN-domains of FlhF involved in flagellar synthesis in Campylobacter jejuni
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NSTL
Elsevier
FlhF protein is critical for intact flagellar assembly in Campylobacter jejuni. It is a putative GTPase with B-, Nand G-domains. However, the role of the Band N-domains in flagella biosynthesis remains unclear in C. jejuni. This study demonstrated that both the Band N-domains are essential for flagellar synthesis, with the absence of B and/or N-domains showing truncated variants of FlhF by TEM. Point mutations in the Band N-domains (T13A, K159A, G231A) also induced flagella abnormalities. Furthermore, significant defects in GTPase activity and polar targeting of FlhF were triggered by point mutations of Band N-domains. Flagella gene expression and transcription were also significantly disrupted in flhF(T13A), flhF(K159A) and flhF(G231A) strains. This study initially explored the effects of Band N-domains on flagella synthesis. We speculated that Band N-domains may directly or indirectly cause flagella abnormalities by affecting flagellar gene expression or GTPase activity, which helps us better understand the function of FlhF in flagella synthesis.
NSTL
Elsevier
