首页|Investigation of major carbapenemase genes in ESBL-producing Escherichia coli and Klebsiella pneumoniae strains isolated from raw milk in Black Sea region of Turkey
Investigation of major carbapenemase genes in ESBL-producing Escherichia coli and Klebsiella pneumoniae strains isolated from raw milk in Black Sea region of Turkey
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NSTL
Elsevier
? 2022 Elsevier LtdThe prevalence of blaKPC, blaNDM, blaOXA-48, blaIMP and blaVIM genes and phenotypic carbapenemase production was investigated in ESBL-producing Enterobacteriaceae strains isolated from 150 raw cow milk samples collected from the Black Sea region of Turkey. A total of 47 phenotypic ESBL producers, 41 Escherichia coli and six Klebsiella pneumoniae, were investigated in terms of carbapenemase activity. The presence of carbapenemase genes was investigated using five single PCR assays. Seven of the 47 isolates were found resistant to imipenem and/or meropenem. PCR assays revealed that 33 of the 47 isolates were found to harbour at least one of the analysed carbapenemase genes. For blaKPC, blaOXA-48, blaIMP and blaVIM genes, BLAST search indicated 97.9%, 97.2%, 97.1% and 97% nucleotide identity to corresponding sequences, respectively. Carbapenemase-producing E. coli and K. pneumoniae were found higher in milk compared with studies conducted worldwide and carbapenemase production was associated with blaOXA-48 and blaKPC.
Uyanik T.、Cadirci O.、Gucukoglu A.、Can C.
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Ondokuz May?s University Faculty of Veterinary Medicine Department of Food Hygiene and Technology
Ondokuz May?s University Faculty of Sciences Department of Molecular Biology and Genetics
NSTL
Elsevier
