首页|miR-326 functions as a tumor suppressor in human prostatic carcinoma by targeting Mucin1

miR-326 functions as a tumor suppressor in human prostatic carcinoma by targeting Mucin1

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  • NSTL
  • Elsevier
Accumulating evidence suggests that microRNA-326 (miR-326) serves as a tumor suppressor in the initiation and progression of several human malignancies。 However， the biological function and underlying molecular mechanism of miR-326 in prostatic carcinoma (PCa) remains largely unknown。 In the present study， we found that miR-326 expression level was significantly downregulated in both primary PCa and castration-resistant PCa (CRPC) tissue samples as detected by qRT-PCR。 Downregulation of miR-326 was closely associated with aggressive progression and poor prognosis of primary PCa patients。 Gain-and lose-functional experiments revealed that forced expression of miR-326 significantly inhibited cell proliferation， colony formation， migration and invasion， induced G0/G1 cell cycle arrest， and promoted apoptosis in PCa cells in vitro， whereas， knockdown of miR-326 expression showed the opposite results。 Overexpression of miR-326 also suppressed tumor growth in xenografted nude mice in vivo。 Moreover， Luciferase reporter， qRT-PCR， and western blot assays identified that the 3'-untranslated region (3'-UTR) of Mucin1 (MUC1) was a direct target region of miR-326。 Spearman's correlation analysis also confirmed an inverse relationship between miR-326 and MUC1 expressions in primary PCa tissue samples。 In addition， restoration of MUC1 expression effectively abrogated the inhibitory effects of miR-326 on PCa proliferation， invasion and migration through the activation of JNK signaling pathway。 Therefore， these data indicated that miR-326 functioned as a tumor suppressor in PCa by negatively regulating MUC1， and that miR-326 might serve as a potential therapeutic candidate for PCa treatment。

miR-326Mucin1Prostatic carcinomaProliferationInvasion

Liang, Xuan、Li, Zhaolun、Men, Qunli、Li, Yongwei、Li, Hechen、Chong, Tie

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Xi An Jiao Tong Univ, Affiliated Hosp 1, Dept Oncol, 227 Yantai West Rd, Xian 710004, Shaanxi

Xi An Jiao Tong Univ, Affiliated Hosp 2, Dept Urol, Xian 710004, Shaanxi, Peoples R China

Cent Hosp Baoji City, Dept Urol, Baoji 721008, Shaanxi, Peoples R China

Cent Hosp Weinan City, Dept Urol, Weinan 714000, Shaanxi, Peoples R China

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2018

10.1016/j.biopha.2018.09.053

Biomedicine & pharmacotherapy

Biomedicine & pharmacotherapy

SCI
ISSN：0753-3322
年,卷(期)：2018.108
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  • 41