首页|High voltage atmospheric cold plasma modification of bovine serum albumin

High voltage atmospheric cold plasma modification of bovine serum albumin

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  • NSTL
  • Elsevier
While atmospheric cold plasma has successfully inactivated biohazardous proteinaceous molecules (unwanted enzymes, prions, and allergens) and modified proteins with improved functionality, few studies have characterized plasma-protein interactions. This study investigates the physicochemical interactions, structural alteration, and reaction mechanisms of bovine serum albumin (BSA) subjected to high voltage atmospheric cold plasma (HVACP) generated by a dielectric barrier discharge in sealed bags packed with air or modified air (65% O2, 30% N2, 5% CO2). Treating 10 mL of BSA solution (50 mg/mL) with HVACP for 60 min changed the sample from transparent to yellow and induced protein precipitation. HVACP also induced protein unfolding, altered secondary structure (27% loss of alpha-helix), and increased disorder structure (10% increase of random coil). HVACP-treated protein increased in average size from 10 nm to 113 mu m, with a broader size distribution after 60 min of HVACP treatment. SDS-PAGE and mass spectrometry showed the formation of new peptides from 1 to 10 kDa, indicating plasma-triggered peptide bond cleavage. Chemical analysis and mass spectrometry demonstrated oxidation and deamidation in HVACP-treated samples. This study illustrates that interactions between HVACPinduced reactive species and proteins may introduce structural alterations, protein aggregation, peptide cleavage, and side-group modifications to proteins in aqueous conditions.

Cold plasmaBovine serum albuminProtein modification

Xu, Lei、Hou, Hu、Farkas, Brian、Keener, Kevin M.、Garner, Allen L.、Tao, Bernard

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Purdue Univ, Dept Food Sci, W Lafayette, IN 47907 USA

Ocean Univ China, Coll Food Sci & Engn, Qingdao 266003, Shandong, Peoples R China

Univ Guelph, Sch Engn, Guelph, ON N1G 2W1, Canada

Purdue Univ, Sch Nucl Engn, W Lafayette, IN 47906 USA

Purdue Univ, Dept Agr & Biol Engn, W Lafayette, IN 47907 USA

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2021

10.1016/j.lwt.2021.111995

LWT-Food Science & Technology

LWT-Food Science & Technology

ISSN：0023-6438
年,卷(期)：2021.149
  • 15
  • 95