We report here the efficient synthesis of prenyl-containing i(6)A-RNA bearing the representative ACGCUAAAAA sequence through phosphoramidite chemistry. To determine proof-of-concept, we have developed two approaches for selectively fluorescent labeling of this i(6)A-RNA, one relied on direct use of probe PTAD-DBCO-Cy5 via Ene-ligation in 37% yield, another one constituted two sequential processes including azidation by use of TMSN3/Selectfluor and subsequent click reaction with DBCO-Cy5 in total 89% yield. (C) 2022 Elsevier Ltd. All rights reserved.
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