首页|Kinetochore-microtubule attachment is sufficient to satisfy the human spindle assembly checkpoint
Kinetochore-microtubule attachment is sufficient to satisfy the human spindle assembly checkpoint
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NSTL
Springer Nature
The spindle assembly checkpoint (SAC) is a genome surveillance mechanism that protects against aneuploidization. Despite profound progress on understanding mechanisms of its activation, it remains unknown what aspect of chromosome-spindle interactions is monitored by the SAC: kinetochore-microtubule attachment or the force generated by dynamic microtubules that signals stable biorientation of chromosomes? To answer this, we uncoupled these two processes by expressing a non-phosphorylatable version of the main microtubule-binding protein at kinetochores (HEC1-9A), causing stabilization of incorrect kinetochore-microtubule attachments despite persistent activity of the error-correction machinery. The SAC is fully functional in HEC1-9A-expressing cells, yet cells in which chromosomes cannot biorient but are stably attached to microtubules satisfy the SAC and exit mitosis. SAC satisfaction requires neither intra-kinetochore stretching nor dynamic microtubules. Our findings support the hypothesis that in human cells the end-on interactions of microtubules with kinetochores are sufficient to satisfy the SAC without the need for microtubule-based pulling forces.
ACCURATE CHROMOSOME SEGREGATIONAURORA-BMITOTIC CHECKPOINTUNATTACHED KINETOCHORESERROR-CORRECTIONNDC80 COMPLEXTENSIONMPS1PHOSPHORYLATIONMITOSIS
Etemad, Banafsheh、Kuijt, Timo E. F.、Kops, Geert J. P. L.
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Royal Netherlands Acad Arts & Sci, Hubrecht Inst KNAW, NL-3584 CT Utrecht, Netherlands
NSTL
Springer Nature
