首页|Gelatin methacryloyl-alginate core-shell microcapsules as efficient delivery platforms for prevascularized microtissues in endodontic regeneration

Gelatin methacryloyl-alginate core-shell microcapsules as efficient delivery platforms for prevascularized microtissues in endodontic regeneration

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  • NSTL
  • Elsevier
A B S T R A C T Combined injectable cell-laden microspheres and angiogenesis approaches are promising for functional vascularized endodontic regeneration. However, advanced microsphere designs and production techniques that benefit practical applications are rarely developed. Herein, gelatin methacryloyl (GelMA)-alginate core-shell microcapsules were fabricated to co-encapsulate human dental pulp stem cells (hDPSCs) and human umbilical vein endothelial cells (HUVECs) based on a coaxial electrostatic microdroplet technique. This technique enables high-throughput production, convenient collection, and minimal material waste. The average diameter of core-shell microcapsules was-359 mu m, and that of GelMA cores was-278 mu m. There were higher proliferation rates for hDPSCs and HUVECs co-encapsulated in the GelMA cores than for hDPSCs or HUVECs monoculture group. HUVECs assembled to form 3D capillary-like networks in co-culture microcapsules. Moreover, HUVECs promoted the osteo/odontogenic differentiation of hDP-SCs in microcapsules. After 14 days of cultivation, prevascularized microtissues formed in microcapsules that contained abundant deposited extracellular matrix (ECM); no microcapsule aggregation occurred. In vivo studies confirmed that better microvessel formation and pulp-like tissue regeneration occurred in the co-culture group than in hDPSCs group. Thus, an effective platform for prevascularization microtissue preparation was proposed and showed great promise in endodontic regeneration and tissue engineering applications.

Endodontic regenerationPrevascularizationCore-shell microcapsuleCoaxial electrostatic microdropletCo-culturePULP STEM-CELLSHUMAN DENTAL-PULPENDOTHELIAL-CELLSFIBRONECTIN PROMOTESCOAXIAL ELECTROSPRAYIN-VITROMICROSPHERESHYDROGELVASCULARIZATIONMICROFLUIDICS

Hu, Xingyu、Yang, Ziyang、Tian, Weidong、Liang, Xi、Xie, Li、Zhang, Qingyuan、Wang, Ge、Zhang, Siyuan、Jiang, Mingyan、Zhang, Ruitao、Yang, Ting

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West China Hosp Stomatol,Sichuan Univ

2022

10.1016/j.actbio.2022.03.045

Acta biomaterialia

Acta biomaterialia

EISCI
ISSN：1742-7061
年,卷(期)：2022.144
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