首页|A new peptide-mediated COF nanocatalytic amplification SERS quantitative assay for ultratrace Cu~(2+)
A new peptide-mediated COF nanocatalytic amplification SERS quantitative assay for ultratrace Cu~(2+)
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NSTL
Peptides are an important recognition element for small molecules and have been used in the field of biological analysis. However, it has been rarely reported that a novel method combining peptides as recognition elements with covalent organic framework (COF) as catalytic amplification signal to construct highly sensitive and selective three-mode molecular spectroscopy. In this paper, high catalytic and stable three COFs were prepared, and the catalysis of the new indicator nanoreaction of HAuCl4-sodiumformate (Fo) was studied by molecular spectral slope procedure. The produced gold nanoparticles (AuNPs) exhibit a strong resonance Rayleigh scattering (RRS) peak at 370 nm and surface plasmon resonance absorption (Abs) peak at 540 nm. In the presence of molecular probes, a strongest surface-enhanced Raman scattering (SERS) peak was generated at 1617 cm~(-1). Combining the COF_(TpBD) amplification indicator reaction with specific peptide (FT) reaction of Cu~(2+), a novel, sensitive and selective SERS/RRS/Abs assay platform was established, with a SERS linear range of 0.005-0.115 nmol/L Cu~(2+). In addition, the two working curves of lg[Cu~(2+)] vs SERS and [Cu~(2+)] vs SERS intensity, and the nanocatalytic mechanism were discussed.
NSTL
