首页|Disposable biosensor based on novel ternary Ru-PEI@PCN-333(Al) self-enhanced electrochemiluminescence system for on-site determination of caspase-3 activity

Disposable biosensor based on novel ternary Ru-PEI@PCN-333(Al) self-enhanced electrochemiluminescence system for on-site determination of caspase-3 activity

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  • NSTL
  • Elsevier
The number of death due to cancer-related diseases each year is at the alarming level and is constantly growing. Tools that can effectively and conveniently detect cancer cell apoptosis can play a significant role in cancer research, cancer therapy, and other related industries. Herein, we fabricated, for the first time, an ultrasensitive, disposable, self-enhanced off-on electrochemiluminescence (ECL) biosensor based on ternary Ru-PEI@PCN-333 (Al) system to determine caspase-3 activity, the biomarker of apoptosis. The biosensor had a low detection limit of 0.017 pg/mL and was able to enhance the ECL emission and stability. A solid-state (SS) ECL strategy was adopted to overcome the relatively weak ECL emission due to the long distance between electro-chemiluminophore and electrode surface. The analysis requires only one incubation step, which can significantly reduce the operational complexity and time. The biosensor had higher sensitivity, and the off-on ECL mode was achieved using caspase-3 as a switch. The on-site and rapid detection capability of the biosensor was achieved by the introduction of disposable screen-printed electrodes (SPEs). This study lays a foundation for the development of more advanced, ingenious, portable and reliable ECL devices for biosensing not only caspase-3, but also other bioanalytes.

Caspase-3ApoptosisElectrochemiluminescence (ECL)Metal organic frameworks (MOFs)Screen-printed electrodes (SPEs)Electrochemical sensorsBiosensorMETAL-ORGANIC FRAMEWORKELECTROCHEMICAL APPROACHGOLD NANOPARTICLECELL APOPTOSISPOINTIMMOBILIZATIONGENERATIONPATHWAYS

Luo, Weiwei、Chu, Hongyu、Wu, Xinzhao、Ma, Pinyi、Wu, Qiong、Song, Daqian

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Jilin Univ

2022

10.1016/j.talanta.2021.123083

Talanta

Talanta

EISCI
ISSN：0039-9140
年,卷(期)：2022.239
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