首页|A dual-mode optical assay of Hg(II)ions via simulated oxidase and fluorescence based on composites formed by crosslinking of glutaraldehyde and chitosan-nano-selenium
A dual-mode optical assay of Hg(II)ions via simulated oxidase and fluorescence based on composites formed by crosslinking of glutaraldehyde and chitosan-nano-selenium
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The crosslinking of glutaraldehyde(GA)and nano-selenium coated chitosan(CS-SeNPs)afforded composites(GA-CS-SeNPs)which exhibit new properties.In addition to having mimic enzyme activity of CS-SeNPs,these composites exhibit fluorescence,which CS-SeNPs did not possess.The GA-CS-SeNPs can catalyze the chromo-genie process of oxidation of substrate TMB at 20 ℃ and pH of 3.5,and under the excitation wavelength of 440 nm,the composites can emit strong fluorescence.The presence of mercury(II)(Hg~(2+))inhibited the color process of catalytic oxidation of 3,3,5,5,-tetramethylbenzidine(TMB)and enhanced the fluorescence intensity of the composites to some extent.Therefore,using the fluorescence and mimicking enzyme properties of GA-CS-SeNPs,a fluorescent and colorimetric(dual-mode)determination of Hg~(2+)was developed with good selectivity and high sensitivity.The linear determination ranges of Hg~(2+)were 0-55 μM and 0-50 ^M for fluorescent and colorimetric assays,respectively,and the limits of detection(LOD)for the dual-mode assay were as low as 0.34(iM and 0.40 μM,respectively.In addition,this new method was successfully implemented for dual-mode determination of Hg~(2+)in real samples.
GA-CS-SeNPsFluorescence and colorimetryDual modeHg~(2+)determination
Changfang Lu、Yutong Wang、Wei Zhang
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College of Science,Sichuan Agricultural University,Chengdu,611130,Sichuan,China
College of Veterinary Medicine,Sichuan Agricultural University,Chengdu,611130,Sichuan,China
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