首页|Endogenous bioactive compounds of naked oats (Avena nuda L.) inhibit alpha-amylase and alpha-glucosidase activity

Endogenous bioactive compounds of naked oats (Avena nuda L.) inhibit alpha-amylase and alpha-glucosidase activity

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This study aims to investigate the inhibitory effects of free and bound bioactive extracts from naked oat on amylase and glucosidase activity. The free compound extract mainly included kaempferol-3-O-galactoside-6 ''-rhamnoside-3'''-rha, hesperetin-7-O-neohesperidoside, ginsenoside Re and soyasaponin Ba. Ten substances were detected in the bound compound extract, including ferulic acid, oxyacanthine, and trans-4-coumaric acid. The free and bound compound extracts showed dose-dependent inhibitory effects on alpha-amylase and alpha-glucosidase; the inhibition of alpha-amylase was reversible anti-competitive inhibition, and the inhibition of alpha-glucosidase was reversible linear mixed inhibition. The saponin ginsenoside Re had poor inhibitory effects on alpha-amylase and alpha-glucosidase; the saponin soyasaponin Ba had a poor inhibitory effect on alpha-amylase but a good inhibitory effect on alpha-glucosidase. After adding the extracts to the different starches, the hydrolysis rates all decreased significantly (p < 0.05). This study elucidates the low digestibility of naked oats and will help facilitate the development of low-glycemic foods using naked oats.

Naked oatSoyasaponin BaIn vitro digestibilityalpha-Amylasealpha-Glucosidase

Wei, Mengjie、Tang, Minyue、Wang, Luyu、Cheng, Xuanxuan、Wu, Yanwen、Ouyang, Jie

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Beijing Forestry Univ, Beijing Key Lab Forest Food Proc & Safety, Coll Biol Sci & Technol, Dept Food Sci & Engn, Beijing 100083, Peoples R China

Beijing Acad Sci & Technol, Beijing Food Safety Anal & Testing Engn Res Ctr, Beijing Ctr Phys & Chem Anal, Beijing 100089, Peoples R China

2021

LWT-Food Science & Technology

LWT-Food Science & Technology

ISSN:0023-6438
年,卷(期):2021.149
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