首页|Dirigent gene family is involved in the molecular interaction between Panax notoginseng and root rot pathogen Fusarium solani
Dirigent gene family is involved in the molecular interaction between Panax notoginseng and root rot pathogen Fusarium solani
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NSTL
Elsevier
Root rot, mainly caused by Fusarium solani, severely reduces the yield and quality of Panax notoginseng (Burk) F. H. Chen. Dirigent (DIR) proteins catalyze the coupling of lignin monomers during lignin biosynthesis, making them important components of plant defense systems. In this study, a small P. notoginseng DIR gene family was isolated from P. notoginseng. A phylogenetic analysis indicated that PnDIR1 belongs to the DIR-a subfamily, whereas the other six PnDIRs belong to the DIR-b/d subfamily. The expression patterns determined by real-time PCR revealed that PnDIR transcription was up-regulated by F. solani and modulated by some hormone signals (except PnDIR5). The PnDIR1 was a cell wall localized protein, and the PnDIR1 promoter activity was induced by several plant hormones and multiple stress stimuli. The recombinant PnDIR1 protein inhibited the growth of Sclerotinia sclerotiorum and F. solani in vitro. Moreover, the overexpression of PnDIR1 in tobacco (Nicotiana tabacum) up-regulated the expression of some genes involved in the biosynthesis of lignin and lignan and considerably increased the resistance to F. solani as well as the increase in lignin accumulation. The results of this study suggest that PnDIRs may facilitate the molecular interaction between P. notoginseng and F. solani through be involved in lignin biosynthesis.
NSTL
Elsevier
