Abstract
The genus Segestria Latreille, 1804 includes 21 species distributed mainly in the Palearctic region. Only two species have been described from South America: Segestria ruficeps Guerin, 1832 (known from Brazil, Uruguay and Argentina) and Segestria pusilla Nicolet, 1849 (known only from Chile). A study of the female genitalia shows that S. ruficeps is a junior synonym of the Paleartcic species Segestria florentina (Rossi 1790), which is redescribed and illustrated. S. pusilla is transferred to the genus Ariadna as a nomen dubium.