Identification of seven undescribed cucurbitacins in Cucumis sativus (cucumber) and their cytotoxic activity

Han, Lida ¹Li, Pengkun ²Zha, Ziou ²Liu, Chang ²Liu, Xiubin ³Huang, Peng ³Liu, Yisong ³Tang, Qi ³Zeng, Kewu ⁴Zeng, Jianguo ³Zhou, Yuan ²Qing, Zhixing ³Shi, Yao²

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作者信息

1. Chinese Acad Agr Sci
2. Huazhong Univ Sci & Technol
3. Hunan Agr Univ
4. Peking Univ
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Abstract

Cucurbitacin C-type cucurbitacins that are only identified in Cucumis sativus (cucumber) are, in part, responsible for the health benefits and bitter flavor. Nevertheless, detailed information about those functional ingredients in cucumber is scarce. In this study, ten cucurbitacin C analogues including seven undescribed ones have been isolated from the bitter leaves of cucumber, in which six compounds showed growth inhibition capabilities against tumor cell lines HepG2, A549, DU145 and HCT116. Intriguingly, cucurbitacin C6 and C7 exhibited a significant inhibition effect compared to the positive control taxol (IC50 = 1.86 +/- 0.17 mu M) on HepG2 cell line with IC50 values of 10.06 +/- 0.34 mu M and 4.16 +/- 0.42 mu M, respectively. The mechanism of cucurbitacin-induced apoptosis is likely down-regulating the expression of caspase-related proteins. This work enlarges the knowledge of the cucurbitacins in cucumber and highlights the importance of cucumber as a source of specialized metabolites in the food and medicinal industries.

Key words

Cucumis sativus/Cucurbitaceae/Cucumber/Cucurbitacin/Triterpenoid/Cytotoxic/HepG2/DISCOVERY/APOPTOSIS/INHIBITOR/PATHWAY

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出版年

2022

Phytochemistry

CCR

ISSN：0031-9422

被引量1

参考文献量23

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