首页|Differences in the components of Neolamarckia cadamba from different provenance and the drug resistance reversal activity of characteristic alkaloid
Differences in the components of Neolamarckia cadamba from different provenance and the drug resistance reversal activity of characteristic alkaloid
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NSTL
Elsevier
? 2022 Elsevier B.V.Neolamarckia cadamba (Roxb.) Bosser (Rubiaceae) is a fast-growing and economically important tree in Asia, which has a wide range of biological and pharmacological activities. Using the UPLC-MS fingerprints of the barks of N. cadamba that sampled from 12 different provenance (10 trees per provenance) in a provenance trail, the difference in the components of these samples was visualized. Analyzing the spectrum data and the cytotoxic activity, the relationships of chemical composition and in vitro anti-tumor activity had been established. It was noted that 3α-dihydrocadambine (3a-Dhc), one of the characteristic components in N. cadamba, could bind to the active pocket of P-glycoprotein (P-gp), and then inhibit its efflux activity, leading to reverse the adriamycin (ADR) resistance in MCF7/ADR cells with no toxicity. Taken together, our data pointed out that N. cadamba in different families had high but different contents of cadambine alkaloids, and the UPLC-MS fingerprint could be a powerful tool for guiding the variety breeding, and Sample 12, the provenance from Mengla County, may be a valued source for the best prospect of anti-tumor application. More importantly, 3a-Dhc could be a potential lead for developing a P-gp inhibitor with high potency and low toxicity.
Zhang X.、Xiao S.、Nie M.、Huang J.、Chen Z.、Lin L.、Luo F.
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Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm College of Forestry and Landscape Architecture South China Agricultural University
NSTL
Elsevier
