首页|Application of DNA mini-barcoding and infrared spectroscopy for the authentication of the Italian product "bottarga"

Application of DNA mini-barcoding and infrared spectroscopy for the authentication of the Italian product "bottarga"

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  • NSTL
  • Elsevier
Tuna "Bottarga" is an artisanal food speciality prepared from the roe of Bluefin Tuna (Thunnus thynnus). Due to the recent restriction of Tuna catches fixed by EU, cheaper substitutes from yellowfin tuna (Thunnus albacares) are currently available in the mass distribution. Since the product is sold sliced or grated, morphological distinctive features may be lost, and fraudulent substitution is possible. In this study we tested DNA minibarcoding and infrared spectroscopy as tools to trace its authenticity. A certified chemical standard, fresh (n = 4) and processed (n = 11) ovarian identified by the morphological observation of the tuna catches, commercial bottargasamples (n = 5) and artificial aged aliquots of all these samples (n = 50) were used in the experiments. The results showed that infrared spectroscopy failed in the taxonomic distinction, whereas enabled PLS-DA classification of aged samples (and thus the identification of fresh prepared artisanal products) with model non-error rate in fitting equal to 0.96, and 0.89 in cross-validation. Conversely, DNA mini-barcoding with mtDNA control region, success in species identification (100% maximum identity by the standard comparison BLAST approach against GenBank), regardless the processing state of the product.

TunaFood authenticationBiomolecular analysisPCAPLS-DASeafood

Frigerio, Jessica、Marchesi, Claudio、Magoni, Chiara、Saliu, Francesco、Ballabio, Davide、Consonni, Viviana、De Mattia, Fabrizio、Galli, Paolo、Labra, Massimo、Gorini, Tommaso

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FEM2 Ambiente, Piazza Sci 2, I-20126 Milan, Italy

Univ Brescia, Dept Mech & Ind Engn, Via Branze 38, I-25123 Brescia, Italy

Univ Milano Bicocca, Biotechnol & Biosci Dept, Piazza Sci 2, I-20126 Milan, Italy

Univ Milano Bicocca, Dept Earth & Environm Sci, Piazza Sci 1, I-20126 Milan, Italy

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2021

10.1016/j.lwt.2020.110603

LWT-Food Science & Technology

LWT-Food Science & Technology

ISSN：0023-6438
年,卷(期)：2021.139
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