首页|Genome-wide transcriptional response of contrasting genotypes of industrial crop castor to As(V) stress: Identification of genes and mechanisms associated with As(V) tolerance
Genome-wide transcriptional response of contrasting genotypes of industrial crop castor to As(V) stress: Identification of genes and mechanisms associated with As(V) tolerance
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NSTL
Elsevier
Arsenic (As) is toxic to living organisms at high concentrations. Utilization of an industrial non-edible oilseed crop like Ricinus communis L. (castor) for revegetation and phytoremediation of As-contaminated land can be highly beneficial. Although a lot of research has been conducted on castor, little is known about As toxicity and tolerance mechanisms in this bioenergy crop. In the present study, genome-wide transcriptional analysis was performed using RNA sequencing (RNA-Seq) to identify differentially expressed genes in As-tolerant and As sensitive castor genotypes in response to As(V) treatment. RNA-Seq data showed a differential expression pattern for stress-related genes between WM (tolerant) and GCH 2 (sensitive) genotype in response to 200 mu M As (V) treatment. A total of 19 DEGs (13 up and 6 downregulated) and 7157 DEGs (4093 up and 3064 down regulated) were identified in leaves of 200 mu M As(V)-treated WM and GCH 2 genotypes, respectively. Expression of genes associated with metal transport such as ZIP1, ZIP4, ZIP5, NRAMP3, metal chelators (MT-2, MT-3, PCS, NAS), photosynthesis responsive genes corresponding to Chl a-b binding proteins, RuBisCO small subunit and protein low PSII accumulation 3 were downregulated in GCH 2 whereas expression of these genes were either maintained or upregulated in WM in response to 200 mu M toxicity. Expression level of genes involved in As uptake and transport such as phosphate transporters, aquaporins, and mitochondrial dicarboxylate transporter (DIT1, DIT2.1) were upregulated in 200 mu M As(V)-treated sensitive genotype GCH 2 but maintaind in WM.
NSTL
Elsevier
