首页|Near-infrared chemiluminescence imaging of superoxide anion production in kidneys with iron(3+)-nitrilotriacetate-induced acute renal oxidative stress in rats

Near-infrared chemiluminescence imaging of superoxide anion production in kidneys with iron(3+)-nitrilotriacetate-induced acute renal oxidative stress in rats

扫码查看
原文链接
  • NSTL
  • Elsevier
Iron-catalyzed oxidative stress generates reactive oxygen species in the kidney and induces oxidative damage including lipid, protein, and DNA modifications which induces renal injury and may lead to cancer. An analysis of oxidative stress dynamics by reactive oxygen species has not been performed non-invasively in real time in intact kidneys and is a significant challenge in biology and medicine. Here, I report that MCLA-800 is a near-infrared chemiluminescent probe that visualizes the dynamics of superoxide anion (O-2(center dot)-) production and the upstream generation of reactive oxygen species in living rat kidneys suffering acute renal oxidative stress induced by intraperitoneal administration of iron(3+)-nitrilotriacetate (Fe3+-NTA) as a representative Fe3+ chelate. MCLA-800 was intravenously injected at 250 nmol/kg body weight and immediately transported to the kidneys with the emitting light dependent on O-2(center dot)- production. The magnitude of O-2(center dot)- production correlated with the Fe3+-NTA dose. O-2(center dot)- was continuously produced in the blood stream following Fe3+-NTA injection at 0.15 mmol/kg body weight, while peak production in the renal cortex occurred at 24 h, then decreased to the background level at 72 h. This study clearly revealed the dynamics of Fe3+-NTA-mediated O-2(center dot)- production in the living kidney by chemiluminescent imaging of O-2(center dot)- production using MCLA-800.

BioimagingChemiluminescenceKidneyNear-infrared luminescenceOxidative stressReactive oxygen speciesFERRIC-NITRILOTRIACETATEIRON-NITRILOTRIACETATELIPID-PEROXIDATIONCELL CARCINOMANEPHROTOXICITYCOELENTERAZINEPROBECARCINOGENESISMITOCHONDRIALLUMINESCENCE

Teranishi, Katsunori

展开 >

Mie Univ

2022

10.1016/j.jphotobiol.2022.112391

Journal of Photochemistry and Photobiology

Journal of Photochemistry and Photobiology

ISSN：1011-1344
年,卷(期)：2022.228
  • 2
  • 50