Holly (Ilex) is a large genus in Aquifoliaceae, which is comprises of 400 to 600 deciduous and evergreen species. This genus is cultivated as important medicinal and ornamental plants in the temperate and subtropical regions (Galle, 1997; Hu, 1989). The great diversity and adaptability of hollies make them as the king in gardens and landscapes. They can be used as shade trees, dividing lines, hedges, and groundcovers. They have beautiful effects of fruits in autumn, masses of evergreen foliage, and bright glistening color of variegated cultivars (Robinson, 1984). Ilex crenata Thunb. is native to eastern China, Japan, Korea, Kuril, Sakhalin, Philippines, and the Himalayas. Now it is widely planted as an ornamental plant in the southeastern US for itsdense evergreen foliage and various forms (Dirr, 2009). Many cultivars have been released for commercial production such as I. crenata (Fastigiata Group) 'Sky Pencil', which is popular in the landscape for its strongly upright habit and lustrous, dark evergreen foliage (Dirr, 2011).