O-Antigens of Azospirillum zeae N7(T), Azospirillum melinis TMCY 0552(T), and Azospirillum palustre B2(T): Structure Elucidation and Analysis of Biosynthesis Genes

Sigida, E. N. Grinev, V. S. Zdorovenko, E. L. Dmitrenok, A. S. Burygin, G. L. Kondurina, N. K. Konnova, S. A. Fedonenko, Y. P.

Russian journal of bioorganic chemistry2022，Vol.48Issue(3) ：10.DOI:10.1134/S1068162022030177

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NSTL
Springer Nature

O-Antigens of Azospirillum zeae N7(T), Azospirillum melinis TMCY 0552(T), and Azospirillum palustre B2(T): Structure Elucidation and Analysis of Biosynthesis Genes

Sigida, E. N. ¹Grinev, V. S. ¹Zdorovenko, E. L. ¹Dmitrenok, A. S. ¹Burygin, G. L. ¹Kondurina, N. K. ²Konnova, S. A. ¹Fedonenko, Y. P.¹

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作者信息

1. Russian Acad Sci
2. Chernyshevsky Saratov State Univ
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Abstract

O-specific polysaccharides have been isolated from lipopolysaccharides of the Azospirillum zeae N7, Azospirillum melinis TMCY 0552, and Azospirillum palustre B2 type bacterial strains. Using one- and two-dimensional H-1- and C-13 NMR spectroscopy and sugar analysis including the determination of the absolute configurations of monosaccharides, it has been found that the isolated polysaccharides consist of branched tetrasaccharide repeating units of the following structure: -> 3)-alpha-L-Rhap2OAc-(1 -> 2)-[beta-D-Glcp-(1 -> 3)]-alpha-L-Rhap-(1 -> 3)-alpha-L-Rhap-(1 ->, which has been previously described for a number of Azospirillum strains assigned to serogroup III. Functions of genes responsible for the biosynthesis of O-antigens have been identified by comparison with the sequences presented in the available databases, and a high level of their homology has been shown.

Key words

Azospirillum/lipopolysaccharide/O-specific polysaccharide/structure of bacterial polysaccharides/O-antigen gene cluster/SP NOV./POLYSACCHARIDE/CONFIGURATION/SOIL

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出版年

2022

Russian journal of bioorganic chemistry

SCI

ISSN：1068-1620

被引量1

参考文献量37

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