首页|Near-infrared photosensitive dyes based on red fluorescence protein chromophore analogue for photodynamic therapy and two-photon fluorescence imaging
Near-infrared photosensitive dyes based on red fluorescence protein chromophore analogue for photodynamic therapy and two-photon fluorescence imaging
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NSTL
In this paper,two photosensitizers RFP-OH and RFP-Ac based on red fluorescent protein chromophore analogue with NIR emission was synthesized for photodynamic therapy(PDT)in A-549 cells and zebrafish.After the introduction of p-hydroxystyrene and acryloyl ester,the emission wavelengths of RFP-OH and RFP-Ac were red-shifted to the NIR band,which were 721 nm and 758 nm,respectively.And the stokes shift of RFP-OH and RFP-Ac was as large as 228 nm and 260 nm,respectively.The singlet oxygen yield of RFP-OH and RFP-Ac was 35.6% and 28.8%,respectively.Tumor cells are rich in biological thiols,such as Cys.After RFP-Ac encountered with Cys,the singlet oxygen yield of the products increased to 34.1%.In addition,DFT calculations showed that RFP-OH can transition to triplet state through intersystem crossing after being excited to singlet state,thereby enhancing the singlet oxygen yield.Both RFP-OH and RFP-Ac showed low dark toxicity(>91.02%),high photo toxicity,and good PDT effects in A-549 cells and zebrafish.More importantly,RFP-OH exhibited superior two-photon fluorescence imaging properties in zebrafish.In a word,considering to the positive results of the experiments,RFP-OH and RFP-Ac had a great potential for PDT in anti-cancer therapy.
Near infrared fluorescence imagingRed fluorescence protein chromophorePhotodynamic therapyTwo-photon fluorescence imaging
Wenhui Xiang、Badi Liu、Ying Qian
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School of Chemistry and Chemical Engineering,Southeast University,Nanjing,211189,China
NSTL
