首页|Lateral flow assays (LFA) for detection of pathogenic bacteria: A small point-of-care platform for diagnosis of human infectious diseases

Lateral flow assays (LFA) for detection of pathogenic bacteria: A small point-of-care platform for diagnosis of human infectious diseases

扫码查看
原文链接
  • NSTL
  • Elsevier
Up-to-date diagnostics is globally improved by point-of-care testing (POCT) analysis and bedside research works. Development in POCT analysis has been provided mostly by forward-looking engineering technology for biosensing and sensing assessments. Lately, lateral flow assays (LFAs) have attracted a lot of interest as a result of their noteworthy benefits including cost-effectiveness, better portability, being operator friendly and rapid detection. This technique has been employed broadly for monitoring diverse biomarkers linked to ultrasensitive detection of pathogenic bacteria, ecological monitoring, consumer protection, and infectious diseases. LFA analyses established on qualitative and optical outcomes have boosted the objectivity and data efficiency of the assessments. Therefore, developing novel methods with the capability of providing reliable and quantitative information regarding a target analyte in a model and preserving the qualities of LFAs is of great necessity. In this review, the main principles of LFAs, challenges, and prospects for more development in this field in sensing pathogenic bacteria have been summarized. Subsequently, visually-read LFAs improvement to further progressive platforms have been explored by considering the prospects of this very flexible method for ultrasensitive detection of pathogenic bacteria. In addition, novel labeling methodologies, electrochemical and optical transducers are described. Also, recent developments in these detection methods elements in combination with other considered approaches have been highlighted.

Point-of-care testing (POCT) diagnosisLateral-flow immunoassay (LFA)Pathogenic bacteriaSensing and biosensing assaysRecognition elementsBACILLUS-ANTHRACIS SPORESCOMPREHENSIVE LABORATORY EVALUATIONESCHERICHIA-COLI O157H7VIBRIO-CHOLERAE O1COMBINED IMMUNOMAGNETIC SEPARATIONMEDIATED ISOTHERMAL AMPLIFICATIONSUSPICIOUS WHITE POWDERSON-SITE DETECTIONRAPID DETECTIONLISTERIA-MONOCYTOGENES

Majidi, Mir Reza、Fakhraei, Mina、Jahanban-Esfahlan, Ali、Hejazi, Maryam、Sohrabi, Hessamaddin、Oroojalian, Fatemeh、Baradaran, Behzad、Tohidast, Maryam、de la Guardia, Miguel、Mokhtarzadeh, Ahad

展开 >

Univ Tabriz

Tabriz Univ Med Sci

Univ Tehran Med Sci

North Khorasan Univ Med Sci

Univ Valencia

展开 >

2022

10.1016/j.talanta.2022.123330

Talanta

Talanta

EISCI
ISSN：0039-9140
年,卷(期)：2022.243
  • 24
  • 134